Difference between revisions of "Part:BBa K1140002"
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<partinfo>BBa_K1140002 short</partinfo> | <partinfo>BBa_K1140002 short</partinfo> | ||
− | The gene is under the regulation of a pLac promoter and a thermolabile | + | The gene is under the regulation of a pLac promoter and a thermolabile RBS [3] that should allow for translation only at temperatures above 32°C. At the 3’ end of the gene dubbed “GFP-LVA” are two consecutive transcription termination regions. |
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Latest revision as of 04:55, 26 September 2013
pLac + 32 oC RNA thermometer + GFP(LVA)
The gene is under the regulation of a pLac promoter and a thermolabile RBS [3] that should allow for translation only at temperatures above 32°C. At the 3’ end of the gene dubbed “GFP-LVA” are two consecutive transcription termination regions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 797