Difference between revisions of "Part:BBa K1031014"
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cI protein,which is a transcription factor,is a part of bandpass filter.It can repress the hybrid promoter K1031021[https://parts.igem.org/Part:BBa_K1031021].In order to fine-tune the band-pass filter,we construct a libiary of rbs before cI protein.
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== '''Components and Characterization''' ==
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===Usage and Biology===
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K1031014 consists of NahR[https://parts.igem.org/Part:BBa_J61051], Psal promoter, RBS B0031[https://parts.igem.org/Part:BBa_B0031] and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter[https://parts.igem.org/Part:BBa_K1031021]. It is the repression branch of the Band-pass Filter circuit ('''Fig 1'''). In order to fine-tune the expression level of transcriptional repressor, we adjusted the RBS (Ribosome Binding Site) intensity in front of CƖ. K1031014 adopts RBS B0031. 
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/4/43/Peking2013_part_K1031013.png/800px-Peking2013_part_K1031013.png", width=600px; />
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'''Fig 1''' Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once Psal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP. The RBS B0031 is shown in light green, regulating the expression level of CƖ protein. 
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== '''Sequence and Features''' ==
 
== '''Sequence and Features''' ==
 
<partinfo>BBa_K1031014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031014 SequenceAndFeatures</partinfo>
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== '''Data shown''' ==
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<img src=", width=600px; />
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'''Fig 2''' Induction ratio of repression circuit adopting RBS B0031 when exposed to inducers. Horizontal axis represents salicylic acid at different concentration. Vertical axis stands for induction ratio calculated from fluorescence intensity.
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1031014 parameters</partinfo>
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<partinfo>BBa_K1031011 parameters</partinfo>
 
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Revision as of 19:54, 25 September 2013

NahR-Psal-B0031-RiboJ-CI


Components and Characterization

K1031014 consists of NahR[1], Psal promoter, RBS B0031[2] and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter[3]. It is the repression branch of the Band-pass Filter circuit (Fig 1). In order to fine-tune the expression level of transcriptional repressor, we adjusted the RBS (Ribosome Binding Site) intensity in front of CƖ. K1031014 adopts RBS B0031.

Fig 1 Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once Psal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP. The RBS B0031 is shown in light green, regulating the expression level of CƖ protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]



Data shown