Difference between revisions of "Part:BBa K1031013"
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cI protein,which is a transcription factor,is a part of bandpass filter.It can repress the hybrid promoter K1031021[https://parts.igem.org/Part:BBa_K1031021].In order to fine-tune the band-pass filter,we construct a libiary of rbs before cI protein.
 
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===Usage and Biology===
 
  
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== '''Component and Characterization''' ==
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K1031013 consists of NahR[https://parts.igem.org/Part:BBa_J61051], pSal promoter and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter[https://parts.igem.org/Part:BBa_K1031021]. It is the repression branch of the Band-pass Filter circuit ('''Fig 1''').
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/4/43/Peking2013_part_K1031013.png/800px-Peking2013_part_K1031013.png", width=600px; />
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'''Fig 1''' Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating pSal promoter shown in light blue. Once pSal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP. 
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== '''Sequence and Features''' ==
 
== '''Sequence and Features''' ==
 
<partinfo>BBa_K1031013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031013 SequenceAndFeatures</partinfo>
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== '''Data shown''' ==
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Previous work done by Dr. Lou Chunbo showed CƖ had obvious inhibiting effect to downstream promoter. ('''Fig 2''').
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/3/35/Peking2013_part_C1.jpg/800px-Peking2013_part_C1.jpg", width=600px; />
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'''Fig 2''' Induction ratio of repression circuit when exposed to inducers. Horizontal axis represents salicylic acid at different concentration. Vertical axis stands for induction ratio calculated from fluorescence intensity. (Data from Dr.Lou Chunbo)
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1031013 parameters</partinfo>
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<partinfo>BBa_K1031011 parameters</partinfo>
 
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Revision as of 19:27, 25 September 2013

NahR-Psal-RBS-RiboJ-CI



Component and Characterization

K1031013 consists of NahR[1], pSal promoter and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter[2]. It is the repression branch of the Band-pass Filter circuit (Fig 1).

Fig 1 Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating pSal promoter shown in light blue. Once pSal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibit hybrid promoter marked with two colors, thus inhibit expression of sfGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]



Data shown

Previous work done by Dr. Lou Chunbo showed CƖ had obvious inhibiting effect to downstream promoter. (Fig 2).

Fig 2 Induction ratio of repression circuit when exposed to inducers. Horizontal axis represents salicylic acid at different concentration. Vertical axis stands for induction ratio calculated from fluorescence intensity. (Data from Dr.Lou Chunbo)