Difference between revisions of "Part:BBa K1088011"

Revision as of 17:48, 25 September 2013

B. subtilis dxs (lac promoter without lac inhibitor)

This part has the dxs gene derived from B. subtilis (BBa_K1088000) controlled by the common Lac promoter. Notice that overexpression of LacI is required for regulation of the lac promoter.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1279
Illegal EcoRI site found at 1936
Illegal PstI site found at 1337
Illegal PstI site found at 1783
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1279
Illegal EcoRI site found at 1936
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1279
Illegal EcoRI site found at 1936
Illegal PstI site found at 1337
Illegal PstI site found at 1783
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1279
Illegal EcoRI site found at 1936
Illegal PstI site found at 1337
Illegal PstI site found at 1783
Illegal NgoMIV site found at 1236
Illegal AgeI site found at 1129
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1078
Illegal SapI.rc site found at 1777

Revision as of 17:45, 25 September 2013 (view source) P.R.A (Talk \| contribs) ← Older edit		Revision as of 17:48, 25 September 2013 (view source) P.R.A (Talk \| contribs) Newer edit →
Line 2:		Line 2:
	<partinfo>BBa_K1088011 short</partinfo>		<partinfo>BBa_K1088011 short</partinfo>

−	This part has the B. ~~sub dxs~~ (BBa_K1088000) controlled by the common Lac promoter. Notice that ~~there~~ overexpression of LacI is required for regulation of the ~~Lac~~ promoter.	+	This part has the ''dxs'' gene derived from ''B. subtilis'' (BBa_K1088000) controlled by the common Lac promoter. Notice that overexpression of LacI is required for regulation of the lac promoter.