Difference between revisions of "Part:BBa K1017811"
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<partinfo>BBa_K1017811 short</partinfo>
 
<partinfo>BBa_K1017811 short</partinfo>
  
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Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to tune the expression level of express part and we choose J23101. rRBS-2 is a sRNA binding site, when sRNA bind on it, this part will be shut down. mRFP(E1010) is a red fluorescent protein. J61048 is a terminator.
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Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to tune the expression level of express part and we choose J23101. rRBS-2 is a sRNA binding site that designed by iGEM13_NCTU_Formosa. When the sRNA from BBa_K1017402 bind on it, this part will be shut down. mRFP(E1010) is a red fluorescent protein. J61048 is a terminator.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:27, 25 September 2013

Pcons+rRBS-2+mRFP+J61048

Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to tune the expression level of express part and we choose J23101. rRBS-2 is a sRNA binding site that designed by iGEM13_NCTU_Formosa. When the sRNA from BBa_K1017402 bind on it, this part will be shut down. mRFP(E1010) is a red fluorescent protein. J61048 is a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 784
    Illegal AgeI site found at 625
    Illegal AgeI site found at 737
  • 1000
    COMPATIBLE WITH RFC[1000]