Difference between revisions of "Part:BBa K1031803"

Revision as of 15:25, 25 September 2013

Pu-B0031-sfGFP-Terminator (XylR)

Structure

Fig 1

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 167
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 219

Construction and tunning

Pc promoter J23114 is selected to initiate the transcription of XylR. Based on this circuit, we constructed a library of RBS (Ribosome Binding Site) including B0031[1], B0032[2], B0033[3] and B0034[4] for tunning for expression level of reporter gene sfGFP (Fig 2). K1031803 consists of Pu promoter, RBS B0031 and reporter gene sfGFP

Fig 2 Construction of reporter circuit. The orange arrow represents Pu promoter for XylR. The green oval stands for RBS B0031. sfGFP coding sequence is shown with dark blue, while terminator B0015[5] is in dark red.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1031803 short</partinfo>
-Pu-31-sfGFP-TT (XylR)
+<!-- -->
+== '''Structure''' ==
+<html>
+<img src="", width=700px; />
+</html>
+'''Fig 1'''
+== '''Sequence and Features''' ==
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1031803 SequenceAndFeatures</partinfo>
+== '''Construction and tunning''' ==
+''Pc'' promoter J23114 is selected to initiate the transcription of XylR. Based on this circuit, we constructed a library of RBS (Ribosome Binding Site) including B0031[https://parts.igem.org/Part:BBa_B0031], B0032[https://parts.igem.org/Part:BBa_B0032], B0033[https://parts.igem.org/Part:BBa_B0033] and B0034[https://parts.igem.org/Part:BBa_B0034] for tunning for expression level of reporter gene sfGFP ('''Fig 2'''). K1031803 consists of Pu promoter, RBS B0031 and reporter gene sfGFP
+<html>
+<img src="https://static.igem.org/mediawiki/igem.org/3/3a/Peking2013_part_31-Pu_XylR.png", width=400px; />
+</html>
+'''Fig 2''' Construction of reporter circuit. The orange arrow represents Pu promoter for XylR. The green oval stands for RBS B0031. sfGFP coding sequence is shown with dark blue, while terminator B0015[https://parts.igem.org/Part:BBa_B0015] is in dark red.
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K1031803 parameters</partinfo>
+<partinfo>BBa_K1035201521 parameters</partinfo>
 <!-- -->