Difference between revisions of "Part:BBa J23117:Experience"
(→Evaluation of Anderson promoter J23117 in E. coli by iGEM Göttingen 2013) |
(→Evaluation of Anderson promoter J23117 in E. coli by iGEM Göttingen 2013) |
||
| Line 36: | Line 36: | ||
The promoter strength was measured by using the reporter gene ''rfp''.<br /> | The promoter strength was measured by using the reporter gene ''rfp''.<br /> | ||
| − | Three different approaches were used: 1. RFP measurement, 2. qRT_PCR analyses and 3. single cell microscopy. Moreover, the first and the second approach characterised the promoter activity along the growth curve and to three important time points, respectively.<br /> | + | Three different approaches were used: '''1. RFP measurement''', '''2. qRT_PCR analyses''' and '''3. single cell microscopy'''. Moreover, the first and the second approach characterised the promoter activity along the growth curve and to three important time points, respectively.<br /> |
Our results from these approaches showed that [[Part:BBa_J23117|BBa_J23177]] has the lowest promoter activity compared to [[Part:BBa_J23116|BBa_J23116]], [[Part:BBa_J23110|BBa_J23110]] and [[Part:BBa_J23118|BBa_J23118]]. | Our results from these approaches showed that [[Part:BBa_J23117|BBa_J23177]] has the lowest promoter activity compared to [[Part:BBa_J23116|BBa_J23116]], [[Part:BBa_J23110|BBa_J23110]] and [[Part:BBa_J23118|BBa_J23118]]. | ||
| Line 47: | Line 47: | ||
on the left side: bright field (BR), on the right side: RFP filter (rfp) <br /> | on the left side: bright field (BR), on the right side: RFP filter (rfp) <br /> | ||
P1: [[Part:BBa_J23117|BBa_J23117]], P2: [[Part:BBa_J23116|BBa_J23116]], P3: [[Part:BBa_J23110|BBa_J23110]], P4: [[Part:BBa_J23118|BBa_J23118]], P8: control ]] | P1: [[Part:BBa_J23117|BBa_J23117]], P2: [[Part:BBa_J23116|BBa_J23116]], P3: [[Part:BBa_J23110|BBa_J23110]], P4: [[Part:BBa_J23118|BBa_J23118]], P8: control ]] | ||
| + | |||
| + | |||
| + | |||
| + | ---- | ||
Revision as of 14:57, 25 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J23117
Evaluation of Anderson promoter J23117 in B. subtilis by iGEM-Team LMU-Munich 2012
This Anderson promoter was evaluated without fused RFP with the lux operon as a reporter in B. subtilis. See the new BioBrick BBa_K823013 without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in B. subtilis.
User Reviews
UNIQf288ccc33c47ab46-partinfo-00000000-QINU UNIQf288ccc33c47ab46-partinfo-00000001-QINU
Evaluation of Anderson promoter J23117 in E. coli by [http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]
Shown here:
Upper two pictures: Growth curves of promoter strains on the left, growth curves of control strains on the right. Three biological replicates are shown.
Middle two pictures: RFP/OD600 of promoter strains on the left, RFP/OD600 of control strains on the right. Three biological replicates are shown.
Bottom three pictures: qRT PCR promoter analyses in three different growth phases. Promoters are normalised against BBa_J23117 .
Promotor 1:BBa_J23117
Promoter 2:BBa_J23116
Promoter 3:BBa_J23110
Promoter 4:BBa_J23118
The promoter strength was measured by using the reporter gene rfp.
Three different approaches were used: 1. RFP measurement, 2. qRT_PCR analyses and 3. single cell microscopy. Moreover, the first and the second approach characterised the promoter activity along the growth curve and to three important time points, respectively.
Our results from these approaches showed that BBa_J23177 has the lowest promoter activity compared to BBa_J23116, BBa_J23110 and BBa_J23118.
on the left side: bright field (BR), on the right side: RFP filter (rfp)
P1: BBa_J23117, P2: BBa_J23116, P3: BBa_J23110, P4: BBa_J23118, P8: control