Difference between revisions of "Part:BBa J23117:Experience"

Revision as of 14:40, 25 September 2013

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Applications of BBa_J23117

Evaluation of Anderson promoter J23117 in B. subtilis by iGEM-Team LMU-Munich 2012

This Anderson promoter was evaluated without fused RFP with the lux operon as a reporter in B. subtilis. See the new BioBrick BBa_K823013 without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in B. subtilis.

User Reviews

UNIQ7038cddf84510f5a-partinfo-00000000-QINU UNIQ7038cddf84510f5a-partinfo-00000001-QINU

Evaluation of Anderson promoter J23117 in E. coli by [http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]

Shown here:
Upper two pictures: Growth curves of promoter strains on the left, growth curves of control strains on the right. Three biological replicates are shown.
Middle two pictures: RFP/OD600 of promoter strains on the left, RFP/OD600 of control strains on the right. Three biological replicates are shown.
Bottom three pictures: qRT PCR promoter analyses in three different growth phases. Promoters are normalised against BBa_J23117 .

Promotor 1:BBa_J23117
Promoter 2:BBa_J23116
Promoter 3:BBa_J23110
Promoter 4:BBa_J23118

The promoter strength was measured by using the reporter gene rfp.
Three different approaches were used: 1. RFP measurement, 2. qRT_PCR analyses and 3. single cell microscopy.
Our results from these approaches showed that BBa_J23177 has the lowest promoter activity compared to BBa_J23116, BBa_J23110 and BBa_J23118.

Fig. 1. RFP and qRT-PCR promoter analyses.

Fig. 2. Microscopic promoter analyses on single cell level
P1: BBa_J23117, P2: BBa_J23116, P3: BBa_J23110, P4: BBa_J23118, P8: control

Revision as of 14:40, 25 September 2013 (view source) Gundl (Talk \| contribs) (→‎Evaluation of Anderson promoter J23117 in E. coli by iGEM Göttingen 2013) ← Older edit		Revision as of 14:40, 25 September 2013 (view source) Gundl (Talk \| contribs) (→‎Evaluation of Anderson promoter J23117 in E. coli by iGEM Göttingen 2013) Newer edit →
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	[[File:promoterGoe.jpg\|420px\|thumb\|right\|'''Fig. 2. Microscopic promoter analyses on single cell level''' <br />		[[File:promoterGoe.jpg\|420px\|thumb\|right\|'''Fig. 2. Microscopic promoter analyses on single cell level''' <br />
−	P1: [[Part:BBa_J23117\|BBa_J23117]], P2: [[Part:BBa_J23116\|BBa_J23116]], P3: [[Part:BBa_J23110\|BBa_J23110]], P4: [[Part:BBa_J23118\|BBa_J23118]], P5: control ]]	+	P1: [[Part:BBa_J23117\|BBa_J23117]], P2: [[Part:BBa_J23116\|BBa_J23116]], P3: [[Part:BBa_J23110\|BBa_J23110]], P4: [[Part:BBa_J23118\|BBa_J23118]], P8: control ]]