Difference between revisions of "Part:BBa K1157021:Experience"
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===Applications of BBa_K1157021=== | ===Applications of BBa_K1157021=== | ||
| + | This construct is a positive feedback circuit that expresses both C-4 AHL receptor RhlR and green fluorescence protein when AHL-binded RhlR combines to its promoter sequence. The design indicates that the promoter sequence is placed in front of the circuit, while the constitutive promoter is placed in the middle of the circuit. We used ELISA plate reader to read GFP fluorescence intensity against C-4 AHL throughout time. The biosensor is cultured overnight and diluted until OD=0.1. We detect the fluorescence intensity from 0 to 4 hours. The results are as follows.[[File:Rhl positive feedback circuit]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:27, 25 September 2013
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Applications of BBa_K1157021
This construct is a positive feedback circuit that expresses both C-4 AHL receptor RhlR and green fluorescence protein when AHL-binded RhlR combines to its promoter sequence. The design indicates that the promoter sequence is placed in front of the circuit, while the constitutive promoter is placed in the middle of the circuit. We used ELISA plate reader to read GFP fluorescence intensity against C-4 AHL throughout time. The biosensor is cultured overnight and diluted until OD=0.1. We detect the fluorescence intensity from 0 to 4 hours. The results are as follows.File:Rhl positive feedback circuit
User Reviews
UNIQ857f4df8bd21a1e1-partinfo-00000000-QINU UNIQ857f4df8bd21a1e1-partinfo-00000001-QINU