Difference between revisions of "Part:BBa K1088007"
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<partinfo>BBa_K1088007 short</partinfo>
 
<partinfo>BBa_K1088007 short</partinfo>
  
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This composite part consists of 5 subparts:
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This part consist of the ''dxs'' gene derived from ''E. coli'' fused to GFP at the translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the lac promoter and has a strong RBS.
  
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A Lac promoter: BBa_R0010
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The purpose of the part was to test the expression profile of ''dxs'' from the lac promter. BBa_K1088012 is a similar part that does not contain the linker-GFP part.
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A strong RBS: BBa_J15001
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The Dxs protein from E. coli: BBa_K118000
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This part is constitutively active when the lac repressor, LacI, isn't overexpressed.
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A 10 aa protein linker: BBa_K105012
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A GFP reporter protein: BBa_E0040
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:25, 25 September 2013

E. coli dxs-GFP fusion (lac promoter without lac inhibitor: IPTG uninducible)

This part consist of the dxs gene derived from E. coli fused to GFP at the translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the lac promoter and has a strong RBS.

The purpose of the part was to test the expression profile of dxs from the lac promter. BBa_K1088012 is a similar part that does not contain the linker-GFP part.

This part is constitutively active when the lac repressor, LacI, isn't overexpressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2756
    Illegal SapI.rc site found at 946