Difference between revisions of "Part:BBa K1045015"

Revision as of 12:17, 25 September 2013

RBS BBa_B0034 with inversed Pre- and Suffix- Promoter reverse - Promoter - DarR operator - BBa_E0240

This part consists of BBa_K1045014 (a weak, inverted promoter upstream of a GFP transcription unit with a strong promoter and the DarR operator). This part also contains an inverse, strong RBS (BBa_K1045010) upstream of the inverse promoter. This part could be used to clone an inverse protein coding sequence upstream of the inverse RBS allowing it to be expressed from the weak promoter, while GFP is expressed from a strong promoter. In addition, GFP expression could be controlled by DarR (BBa_K1045001), if present.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 21
Illegal NheI site found at 44
Illegal NheI site found at 124
Illegal NheI site found at 147
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 75
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 845

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1045015 short</partinfo>
-his part consists of BBa_K1045014 (a weak, inverted promoter upstream of a GFP transcription unit with a strong promoter and the DarR operator). This part contains, as well, an inverse, strong RBS (BBa_K1045010) upstream of the inverse promoter. This part could be used to clone an inverse protein coding sequence upstream of the inverse RBS allowing it to be expressed from the weak promoter, while GFP is expressed from a strong promoter. In addition, GFP expression could be controlled by DarR (BBa_K1045001), if present.
+This part consists of [[Part:BBa_K1045014|BBa_K1045014]] (a weak, inverted promoter upstream of a GFP transcription unit with a strong promoter and the DarR operator). This part also contains an inverse, strong RBS ([[Part:BBa_K1045010|BBa_K1045010]]) upstream of the inverse promoter. This part could be used to clone an inverse protein coding sequence upstream of the inverse RBS allowing it to be expressed from the weak promoter, while GFP is expressed from a strong promoter. In addition, GFP expression could be controlled by DarR ([[Part:BBa_K1045001|BBa_K1045001]]), if present.
 <!-- Add more about the biology of this part here