Difference between revisions of "Part:BBa I13543"
 
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===Usage and Biology===
 
===Usage and Biology===
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Can also be used as an RFP only control, to compare to measurements such as in pSB1A10.  Transcription must be induced off of Pbad, so this part should be used strain CW2553/pJat8 and induced at a high arabinose concentration such as 3e-4%.
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Can also be used as an RFP only control, to compare to measurements such as in pSB1A10.  Transcription must be induced off of Pbad, so this part should be used in strain CW2553/pJat8 and induced at a high arabinose concentration such as 3e-4%.
 
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Latest revision as of 02:57, 18 July 2006

Intermediate in two promoter screening plasmid

-- No description --

Usage and Biology

Can also be used as an RFP only control, to compare to measurements such as in pSB1A10. Transcription must be induced off of Pbad, so this part should be used in strain CW2553/pJat8 and induced at a high arabinose concentration such as 3e-4%.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1325
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1303
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1910
    Illegal AgeI site found at 2022
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961