Difference between revisions of "Part:BBa J23117:Experience"
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| − | ==== | + | ====Evaluation of Anderson promoter J23117 in ''E. coli'' by [http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]==== |
| − | + | <br /><br /> | |
Shown here: <br /> | Shown here: <br /> | ||
'''Upper two pictures:''' Growth curves of promoter strains on the left, growth curves of control strains on the right. Three biological replicates are shown. <br /> | '''Upper two pictures:''' Growth curves of promoter strains on the left, growth curves of control strains on the right. Three biological replicates are shown. <br /> | ||
'''Middle two pictures:''' RFP/OD600 of promoter strains on the left, RFP/OD600 of control strains on the right. Three biological replicates are shown. <br /> | '''Middle two pictures:''' RFP/OD600 of promoter strains on the left, RFP/OD600 of control strains on the right. Three biological replicates are shown. <br /> | ||
| − | '''Bottom three pictures:''' qRT PCR promoter analyses in three different growth phases. Promoters are normalised against BBa_J23117. <br /> | + | '''Bottom three pictures:''' qRT PCR promoter analyses in three different growth phases. Promoters are normalised against [[Part:BBa_J23117|BBa_J23117]] . <br /> |
| − | Promotor 1: | + | <br /> |
| − | Promoter 2: | + | Promotor 1:[[Part:BBa_J23117|BBa_J23117]] <br /> |
| − | Promoter 3: | + | Promoter 2:[[Part:BBa_J23116|BBa_J23116]] <br /> |
| − | Promoter 4: | + | Promoter 3:[[Part:BBa_J23110|BBa_J23110]] <br /> |
| + | Promoter 4:[[Part:BBa_J23118|BBa_J23118]] | ||
[[File:J23117.jpg]] | [[File:J23117.jpg]] | ||
Revision as of 10:53, 25 September 2013
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Applications of BBa_J23117
Evaluation of Anderson promoter J23117 in B. subtilis by iGEM-Team LMU-Munich 2012
This Anderson promoter was evaluated without fused RFP with the lux operon as a reporter in B. subtilis. See the new BioBrick BBa_K823013 without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in B. subtilis.
User Reviews
UNIQe22e3ed775a51ee4-partinfo-00000000-QINU UNIQe22e3ed775a51ee4-partinfo-00000001-QINU
Evaluation of Anderson promoter J23117 in E. coli by [http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]
Shown here:
Upper two pictures: Growth curves of promoter strains on the left, growth curves of control strains on the right. Three biological replicates are shown.
Middle two pictures: RFP/OD600 of promoter strains on the left, RFP/OD600 of control strains on the right. Three biological replicates are shown.
Bottom three pictures: qRT PCR promoter analyses in three different growth phases. Promoters are normalised against BBa_J23117 .
Promotor 1:BBa_J23117
Promoter 2:BBa_J23116
Promoter 3:BBa_J23110
Promoter 4:BBa_J23118
