Difference between revisions of "Part:BBa K1139201"
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We improved a phosphate sensor part since the existing phosphate sensor part (OUC-China 2012, [https://parts.igem.org/Part:BBa_K737024 BBa_K737024]) did not have sufficient data. We constructed this part by amplifying the phoA promoter region of ''E. coli'' (MG1655) and ligating it upstream of GFP part (Fig. 1). This phoA promoter is the inducible promoter of the alkaline phosphatase gene (phoA) from ''E. coli'' (M. Dollard et al., 2003). This promoter is repressed by high concentration phosphate (H. Shinagawa et al., 1983, Y. Hsieh et al., 2010) (Fig. 2).
 
We improved a phosphate sensor part since the existing phosphate sensor part (OUC-China 2012, [https://parts.igem.org/Part:BBa_K737024 BBa_K737024]) did not have sufficient data. We constructed this part by amplifying the phoA promoter region of ''E. coli'' (MG1655) and ligating it upstream of GFP part (Fig. 1). This phoA promoter is the inducible promoter of the alkaline phosphatase gene (phoA) from ''E. coli'' (M. Dollard et al., 2003). This promoter is repressed by high concentration phosphate (H. Shinagawa et al., 1983, Y. Hsieh et al., 2010) (Fig. 2).
  
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[[Image:|thumb|left|500px|'''Fig. 1.''' Our improved part: BBa_ K1139201]][[Image:|thumb|right|500px|'''Fig. 2.''' Mechanism of ''phoA'' promoter]]
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[[Image:Titech2013_parts_K1139201_1.jpg|thumb|center|500px|'''Fig. 1.''' Our improved part: BBa_ K1139201]]
  
  

Revision as of 10:34, 25 September 2013

PphoA-GFP-TT

We constructed this part by ligating PphoA promoter (BBa_K1139200) to the upstream of promoterless GFP generator (BBa_I751310).

We improved a phosphate sensor part since the existing phosphate sensor part (OUC-China 2012, BBa_K737024) did not have sufficient data. We constructed this part by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part (Fig. 1). This phoA promoter is the inducible promoter of the alkaline phosphatase gene (phoA) from E. coli (M. Dollard et al., 2003). This promoter is repressed by high concentration phosphate (H. Shinagawa et al., 1983, Y. Hsieh et al., 2010) (Fig. 2).

Fig. 1. Our improved part: BBa_ K1139201


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 754