Difference between revisions of "Part:BBa K1139201"

Revision as of 10:08, 25 September 2013

PphoA-GFP-TT

We constructed this part by ligating PphoA promoter (BBa_K1139200) to the upstream of promoterless GFP generator (BBa_I751310).

We improved a phosphate sensor part since the existing phosphate sensor part (OUC-China 2012, BBa_K737024) did not have sufficient data. We constructed this part by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part (Fig. 1). This phoA promoter is the inducible promoter of the alkaline phosphatase gene (phoA) from E. coli (M. Dollard et al., 2003). This promoter is repressed by high concentration phosphate (H. Shinagawa et al., 1983, Y. Hsieh et al., 2010) (Fig. 2).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 754

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 We constructed this part by ligating PphoA promoter ([https://parts.igem.org/Part:BBa_K1139200 BBa_K1139200]) to the upstream of promoterless GFP generator ([https://parts.igem.org/Part:BBa_I751310 BBa_I751310]).
+We improved a phosphate sensor part since the existing phosphate sensor part (OUC-China 2012, [https://parts.igem.org/Part:BBa_K737024 BBa_K737024]) did not have sufficient data. We constructed this part by amplifying the phoA promoter region of ''E. coli'' (MG1655) and ligating it upstream of GFP part (Fig. 1). This phoA promoter is the inducible promoter of the alkaline phosphatase gene (phoA) from ''E. coli'' (M. Dollard et al., 2003). This promoter is repressed by high concentration phosphate (H. Shinagawa et al., 1983, Y. Hsieh et al., 2010) (Fig. 2).
 <!-- Add more about the biology of this part here