Difference between revisions of "Part:BBa K1061011"

 
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<partinfo>BBa_K1061011 short</partinfo>
 
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Ef-1alpha promoter is a strong constitutive promoter  that drive the expression of elongation factor -1&#945;.It has been widely used in constructing mammalian cell-based circuit, especially useful for construction of long-term circuit in some cells including iPS cells and Jurkat cells in which the strong promoter CMV may be silenced.
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Ef-1alpha promoter is a strong constitutive promoter  that drive the expression of elongation factor -1&#945;.It has been widely used in constructing mammalian cell-based circuit, especially useful for construction of long-term circuit in some cells including iPS cells and Jurkat cells in which the strong promoter CMV may be silenced.[1]
  
 
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<partinfo>BBa_K1061011 parameters</partinfo>
 
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<h2>References:</h2>
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<p>[1][1] R.V. Gopalkrishnan,  K.A. Christiansen et al, 1999,Use of the human EF-1α promoter for expression can significantly increase success in establishing stable cell lines with consistent expression: A study using the tetracycline-inducible system in human cancer cells, nucleic acids research,27:4775-4782

Revision as of 09:17, 25 September 2013

EF-1alpha promoter

Ef-1alpha promoter is a strong constitutive promoter that drive the expression of elongation factor -1α.It has been widely used in constructing mammalian cell-based circuit, especially useful for construction of long-term circuit in some cells including iPS cells and Jurkat cells in which the strong promoter CMV may be silenced.[1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 440
  • 1000
    COMPATIBLE WITH RFC[1000]


References:

[1][1] R.V. Gopalkrishnan, K.A. Christiansen et al, 1999,Use of the human EF-1α promoter for expression can significantly increase success in establishing stable cell lines with consistent expression: A study using the tetracycline-inducible system in human cancer cells, nucleic acids research,27:4775-4782