Difference between revisions of "Part:BBa K1045005:Design"
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===Design Notes===
 
===Design Notes===
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To be continued
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This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested pSBC13 backbone.
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Revision as of 09:00, 25 September 2013

YdaO Riboswitch with native RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis DNA and ligated into EcoRI and PstI digested pSBC13 backbone.


Source

To be continued

References

Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965