Difference between revisions of "Part:BBa K1119003"

Revision as of 08:54, 25 September 2013

aceB

aceB codes for malate synthase, native in E. coli K-12 strain BW25113. This part is useful for regulating metabolic pathway. This is enzyme involved in the glyoxylate cycle in plants and bacteria. The glyoxylate enzyme allows plants and bacteria to use fatty acids as a substrate for gluconeogenesis and avoid complete oxiation into CO2. HKUST 2013 team has introduced this prokaryotic gene into mammalian cell to create an artificial futile cycle that increases uptake of fatty acids in the medium.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1382
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1416
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1015

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 <partinfo>BBa_K1119003 short</partinfo>
-aceB codes for malate synthase, native in E. coli strain BW25113. This part is useful for regulating metabolic pathway. This is enzyme involved in the glyoxylate cycle in plants and bacteria. The glyoxylate enzyme allows plants and bacteria to use fatty acids as a substrate for gluconeogenesis and avoid complete oxiation into CO2. HKUST 2013 team has introduced this prokaryotic gene into mammalian cell to create an artificial futile cycle that increases uptake of fatty acids in the medium.
+aceB codes for malate synthase, native in E. coli K-12 strain BW25113. This part is useful for regulating metabolic pathway. This is enzyme involved in the glyoxylate cycle in plants and bacteria. The glyoxylate enzyme allows plants and bacteria to use fatty acids as a substrate for gluconeogenesis and avoid complete oxiation into CO2. HKUST 2013 team has introduced this prokaryotic gene into mammalian cell to create an artificial futile cycle that increases uptake of fatty acids in the medium.
 <!-- Add more about the biology of this part here