Difference between revisions of "Part:BBa K1073029"

Revision as of 07:43, 25 September 2013

Inducible ampicillin resistance cassette + LuxR expression cassette

Induction of the beta lactamase mediated ampicillin resistance by LuxR and specific autoinducer N-3-oxo-hexanoyl-HSL. In complex with the autoinducer molecule N-3-oxo-hexanoyl-HSL, the transcription regulator LuxR binds to specific sequences in the promoter region and enables transcription of ampR. This construct provides all the parts required to express the gene downstream of the inducible promoter. Only LuxI, the synthase producing the autoinducer molecule N-3-oxo-hexanoyl-HSL is missing in the construct. However, N-3-oxo-hexanoyl-HSL can be taken up from the media.

Usage and Biology

The expression rate of the genes downstream of the promoter region can be tuned by the concentration of N-3-oxo-hexanoyl-HSL in the media. In this case the expression of beta lactamase can be induced by adding N-3-oxo-hexanoyl-HSL to the culture broth. Expression of LuxR is regulated by the strong constitutive promoter BBa_J23100 and RBS of medium strength (BBa_B0032).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1098
Illegal NheI site found at 1121
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 07:43, 25 September 2013 (view source) Laura S (Talk \| contribs) ← Older edit		Revision as of 07:43, 25 September 2013 (view source) Laura S (Talk \| contribs) (→‎Usage and Biology) Newer edit →
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	===Usage and Biology===		===Usage and Biology===
−	The expression rate of the genes downstream of the promoter region can be tuned by the concentration of N-3-oxo-hexanoyl-HSL in the media. In this case the expression of beta lactamase can be induced by adding N-3-oxo-hexanoyl-HSL to the culture broth. Expression of LuxR is regulated by the strong constitutive promoter BBa_J23100 and RBS of medium ~~strengh~~ (BBa_B0032).	+	The expression rate of the genes downstream of the promoter region can be tuned by the concentration of N-3-oxo-hexanoyl-HSL in the media. In this case the expression of beta lactamase can be induced by adding N-3-oxo-hexanoyl-HSL to the culture broth. Expression of LuxR is regulated by the strong constitutive promoter BBa_J23100 and RBS of medium strength (BBa_B0032).