Difference between revisions of "Part:BBa K1031113"

 
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<partinfo>BBa_K1031113 short</partinfo>
 
<partinfo>BBa_K1031113 short</partinfo>
  
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Po-31-sfGFP-TT (CapR)
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== '''Structure''' ==
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Po promoter which is activated by CapR, is σ-54 dependent. It is composed of three regions. The two inverted UAS sites are responsible for binding of CapR transcriptional factor. The two IHF binding sites allowing IHF to participate enhance transcription efficiency. -24 and -12 region interact with sigma-54 factor of RNA polymerase, enabling the formation of open complex. ('''Fig 1''')
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<img src="https://static.igem.org/mediawiki/igem.org/b/b3/Peking2013_DmpR_Figure3.png", width=600px; />
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'''Fig 1'''  Po promoter structure. The UAS of this promoter marked in green is combined of two parts in contrast direction to which DmpR binds. The box with yellow background represents IHF binding sites. The box with pink background represents σ54 binding site with -24 region and -12 region marked in red. The G with right angle represents +1 site.
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== '''Sequence and Features''' ==
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1031113 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031113 SequenceAndFeatures</partinfo>
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== '''Construction''' ==
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We constructed a library of RBS (Ribosome Binding Site) of different strength for tuning the expression of reporter sfGFP.
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K1031113 is a reporter circuit composed of three elements, the inducible promoter Po, RBS B0031, and reporter gene sfGFP.  ('''Fig 2''')
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<html>
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<img src="https://static.igem.org/mediawiki/igem.org/1/12/Peking2013_part_31-Po-CapR.png", width=600px; />
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1031113 parameters</partinfo>
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<partinfo>BBa_K1031442 parameters</partinfo>
 
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Revision as of 06:59, 25 September 2013

Po-B0031-sfGFP-Terminator (CapR)


Structure

Po promoter which is activated by CapR, is σ-54 dependent. It is composed of three regions. The two inverted UAS sites are responsible for binding of CapR transcriptional factor. The two IHF binding sites allowing IHF to participate enhance transcription efficiency. -24 and -12 region interact with sigma-54 factor of RNA polymerase, enabling the formation of open complex. (Fig 1)

Fig 1 Po promoter structure. The UAS of this promoter marked in green is combined of two parts in contrast direction to which DmpR binds. The box with yellow background represents IHF binding sites. The box with pink background represents σ54 binding site with -24 region and -12 region marked in red. The G with right angle represents +1 site.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 209


Construction

We constructed a library of RBS (Ribosome Binding Site) of different strength for tuning the expression of reporter sfGFP. K1031113 is a reporter circuit composed of three elements, the inducible promoter Po, RBS B0031, and reporter gene sfGFP. (Fig 2)