Difference between revisions of "Part:BBa K1114411"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1114411 short</partinfo>
 
<partinfo>BBa_K1114411 short</partinfo>
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<br>
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This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part.
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The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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Summary of modifications from original part:
  
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LacZ Alpha with EB fusion sites
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 05:05, 25 September 2013

DVL0_EB
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 251
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 251
    Illegal BamHI site found at 272
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 251
    Illegal XbaI site found at 278
    Illegal SpeI site found at 1
    Illegal SpeI site found at 7
    Illegal SpeI site found at 535
    Illegal PstI site found at 290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 528