Difference between revisions of "Part:BBa K1114409"

Revision as of 04:51, 25 September 2013

DVL0_DG This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.

LacZ in pSB1C3 with DG fusion sites.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 245
Illegal XbaI site found at 272
Illegal SpeI site found at 1
Illegal SpeI site found at 529
Illegal PstI site found at 284
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 245
Illegal SpeI site found at 1
Illegal SpeI site found at 529
Illegal PstI site found at 284
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 245
Illegal BamHI site found at 266
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 245
Illegal XbaI site found at 272
Illegal SpeI site found at 1
Illegal SpeI site found at 529
Illegal PstI site found at 284
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 245
Illegal XbaI site found at 272
Illegal SpeI site found at 1
Illegal SpeI site found at 529
Illegal PstI site found at 284
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 8
Illegal BsaI.rc site found at 522

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1114409 short</partinfo>
+This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part.
+The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
+Summary of modifications from original part:
+Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
 LacZ in pSB1C3 with DG fusion sites.