Difference between revisions of "Part:BBa K1114107"

Revision as of 19:47, 24 September 2013 (view source) THaddock (Talk | contribs) ← Older edit		Latest revision as of 03:47, 25 September 2013 (view source) THaddock (Talk | contribs)
Line 6:		Line 6:
	<br><br>		<br><br>
−	This BCD1 is flanked by two MoClo fusion sites, B on the 5' end and C on the 3' side.	+	This BCD is flanked by two MoClo fusion sites, B on the 5' end and C on the 3' side.
	<br><br>		<br><br>
	The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.		The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

---

Latest revision as of 03:47, 25 September 2013

Bicistronic Design RBS 2, MoClo Format with BC fusion sites

This part is a MoClo adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from Mutalik et al., 2013. It contains two Shine-Dalgarno sequences instead of the standard one in ribosomal binding units such as BBa_B0034. From the library of BCDs, this is a MoClo version of BCD2 (BioFAB # apFAB682) and we obtained the sequence data for this part from the BioFAB.

This BCD is flanked by two MoClo fusion sites, B on the 5' end and C on the 3' side.

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

This is Figure 1A from Mutalik et al., 2013 showing the basic structure of their BCD design. The BCD with its two Shine-Dalgarno motifs (SD1 and SD2) is shown in gray.

Sequence and Features