Difference between revisions of "Part:BBa K1080004:Design"
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No Biobrick restriction sites: PstI, XbaI, SpeI or EcoRI | No Biobrick restriction sites: PstI, XbaI, SpeI or EcoRI | ||
− | <b> Amino Acid Sequence | + | <b> Amino Acid Sequence </b> |
<FONT FACE="courier">VGAVVGLGAL LVATDHQKRR SEQMKSFDGD EKEAVKDYFN TAGFERWRKI YGETDEVNKV QLDIRTGHAQ TVDKVLRWVD EEGSVQGITV ADCGCGTGSL<br> AIQLALRGAA VSASDISAAM ASEAEQRYQQ AVAAGQGKAP KVAPKFEALD LESVKGKYDT VTCLDVMIHY PQDKVDAMIT HLAGLSDRRL IISFAPKTLS<br> YSILKRIGEL FPGPSKATRA YLHREEDVEA ALKRAGFKVT KREMTATSFY FSRLLEAIRE </FONT> | <FONT FACE="courier">VGAVVGLGAL LVATDHQKRR SEQMKSFDGD EKEAVKDYFN TAGFERWRKI YGETDEVNKV QLDIRTGHAQ TVDKVLRWVD EEGSVQGITV ADCGCGTGSL<br> AIQLALRGAA VSASDISAAM ASEAEQRYQQ AVAAGQGKAP KVAPKFEALD LESVKGKYDT VTCLDVMIHY PQDKVDAMIT HLAGLSDRRL IISFAPKTLS<br> YSILKRIGEL FPGPSKATRA YLHREEDVEA ALKRAGFKVT KREMTATSFY FSRLLEAIRE </FONT> |
Revision as of 03:15, 25 September 2013
ChlM
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ChlM Clone: DNA sequence from translation start site:
Translated the DNA sequence into a protein sequence using "Translate" at http://au.expasy.org/tools Then used the translated protein sequence to analyse the protein using "ProtParam" at http://au.expasy.org/tools
No Biobrick restriction sites: PstI, XbaI, SpeI or EcoRI
Amino Acid Sequence
VGAVVGLGAL LVATDHQKRR SEQMKSFDGD EKEAVKDYFN TAGFERWRKI YGETDEVNKV QLDIRTGHAQ TVDKVLRWVD EEGSVQGITV ADCGCGTGSL
AIQLALRGAA VSASDISAAM ASEAEQRYQQ AVAAGQGKAP KVAPKFEALD LESVKGKYDT VTCLDVMIHY PQDKVDAMIT HLAGLSDRRL IISFAPKTLS
YSILKRIGEL FPGPSKATRA YLHREEDVEA ALKRAGFKVT KREMTATSFY FSRLLEAIRE
References and documentation are available.
Please note the modified algorithm for extinction coefficient.
Number of amino acids: 260
Molecular weight: 28533.5
Theoretical pI: 7.12
Amino acid composition: Ala (A) 32 12.3% Arg (R) 17 6.5% Asn (N) 2 0.8% Asp (D) 17 6.5% Cys (C) 3 1.2% Gln (Q) 11 4.2% Glu (E) 19 7.3% Gly (G) 20 7.7% His (H) 5 1.9% Ile (I) 12 4.6% Leu (L) 21 8.1% Lys (K) 19 7.3% Met (M) 5 1.9% Phe (F) 9 3.5% Pro (P) 6 2.3% Ser (S) 16 6.2% Thr (T) 15 5.8% Trp (W) 2 0.8% Tyr (Y) 8 3.1% Val (V) 21 8.1% Pyl (O) 0 0.0% Sec (U) 0 0.0%
(B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1258 Hydrogen H 2019 Nitrogen N 355 Oxygen O 385 Sulfur S 8
Formula: C1258H2019N355O385S8 Total number of atoms: 4025
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 23045 Abs 0.1% (=1 g/l) 0.808, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.803, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is V (Val).
The estimated half-life is:
100 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.87 This classifies the protein as stable.
Aliphatic index: 85.23
Grand average of hydropathicity (GRAVY): -0.267
Source
Chlamydomonas reinhardtii