Difference between revisions of "Part:BBa J176001"

Latest revision as of 03:11, 25 September 2013

flyPCD

Polycomb chromodomain from the Drosophila (fruit fly) Pc protein (a.a. 16-78).
Alias: KAH03

Usage and Biology

Drosophila PCD of Pc1 (orange) Crystal structure reported by Fischle et al. 2011. Grey = H3 histone tail; Ball and stick = trimethylated lysine 27

Protein Data Bank ID 1PDQ
Mammalian expression vector required
Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression

See the human variant BBa_J176000 for more details on the biology of the Polycomb chromodomain.

REFERENCES:

Fischle, W, Wang, Y, Jacobs, SA, Kim, Y, Allis, CD, Khorasanizadeh, S. (2003) Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains. Genes Dev. 17:1870-1881.
Min, J, Zhang, Y, Xu, RM. (2003) Structural basis for the specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27. Genes Dev. 17:1823-1828.
Haynes, KA, Silver, PA. (2011) Synthetic reversal of epigenetic silencing. J Biol Chem. 286:27176-27182.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J176001 short</partinfo>
-Polycomb chromodomain (PCD) from the zebrafish CBX2 protein (a.a. 2-64).<br>
+Polycomb chromodomain from the Drosophila (fruit fly) Pc protein (a.a. 16-78).<br>
-Other names: KAH04
+Alias: KAH03
 ===Usage and Biology===
-* Chassis: mammalian cells
+[[Image:Haynes_Pc1_PDB1PDQ.png|thumb|250px|'''Drosophila PCD of Pc1''' (orange) Crystal structure reported by Fischle et al. 2011. Grey = H3 histone tail; Ball and stick = trimethylated lysine 27 ]]
+* Protein Data Bank ID 1PDQ
 * Mammalian expression vector required
 * Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression<br>
@@ Line 14: / Line 16: @@
 See the human variant <partinfo>BBa_J176000</partinfo> for more details on the biology of the Polycomb chromodomain.
+<br><br><br>
+REFERENCES:
+# Fischle, W, Wang, Y, Jacobs, SA, Kim, Y, Allis, CD, Khorasanizadeh, S. (2003) Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains. Genes Dev. 17:1870-1881.
+# Min, J, Zhang, Y, Xu, RM. (2003) Structural basis for the specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27. Genes Dev. 17:1823-1828.
+# Haynes, KA, Silver, PA. (2011) Synthetic reversal of epigenetic silencing. J Biol Chem. 286:27176-27182.
+<br><br><br><br><br><br><br><br>
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