Difference between revisions of "Part:BBa K1045001"

Revision as of 22:40, 24 September 2013

DarR ORF with inversed Pre- and Suffix

This is the ORF of DarR. DarR is a regulatory protein from Mycobacterium smegmatis, which can bind its specific binding sequence (the DarR_operator, see part BBa_K1045000). The binding effinciency is dependent on the availability of cyclic di-AMP, which enhances the probability of binding to DNA. Ideally, this protein will shut down transcription of information downstream of its operator. This way, transcription can be controlled via the level of c-di-AMP (e.g. for a reporter-system in order to monitor it).

(Reference: Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 581
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 594
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 31
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This way, transcription can be controlled via the level of c-di-AMP (e.g. for a reporter-system in order to monitor it).
-(Reference: Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a CyclicDi-AMP-responsive Repressor in ''Mycobacterium smegmatis''”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096)
+(Reference: Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in ''Mycobacterium smegmatis''”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096)
 <!-- Add more about the biology of this part here