Difference between revisions of "Part:BBa K1140006:Design"
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===Source=== | ===Source=== | ||
| − | Obtained synthetically. | + | Obtained synthetically. |
| + | The RNA thermosensor sequence was obtained and adapted from [1] (u6 synthetic RNA thermometer). | ||
The RFP variant employed is the same that is found in part BBa_E1010, which is a monomeric variant of the red fluorescent protein found in the coral ''Discosoma striata''. | The RFP variant employed is the same that is found in part BBa_E1010, which is a monomeric variant of the red fluorescent protein found in the coral ''Discosoma striata''. | ||
The pTet promoter is identical to the sequence found in part BBa_R0040. | The pTet promoter is identical to the sequence found in part BBa_R0040. | ||
Revision as of 20:40, 24 September 2013
pTet + 37 oC RNA thermometer + mCherry (LVA)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 63
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 676
Illegal AgeI site found at 788 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.
Source
Obtained synthetically. The RNA thermosensor sequence was obtained and adapted from [1] (u6 synthetic RNA thermometer). The RFP variant employed is the same that is found in part BBa_E1010, which is a monomeric variant of the red fluorescent protein found in the coral Discosoma striata. The pTet promoter is identical to the sequence found in part BBa_R0040.
References
Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, Nature, 20;403(6767):335-8.
Neupert J, Karcher D, Bock R (2008) Design of simple synthetic RNA thermometers for temperature- controlled gene expression in Escherichia coli. "Nucleic Acids Res, 36(19):e124.