Difference between revisions of "Part:BBa K1114106:Design"

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MoClo formatted version of B0034 with BC fusion sites
 
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<partinfo>BBa_K1114106 short</partinfo>
 
<partinfo>BBa_K1114106 short</partinfo>

Latest revision as of 20:36, 24 September 2013

Bicistronic Design RBS 1, MoClo Format with BC fusion sites



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.

Source

This part was made by cloning off a BiCistronic Design RBS 2 template synthesized by Sonya Iverson of the CIDAR Group.

References