Difference between revisions of "Part:BBa K1114108:Design"
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| − | This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her | + | This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD8 part off of a paper published by <a href= "http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html">Mutalik, et al.</a> |
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Latest revision as of 19:50, 24 September 2013
Bicistronic design RBS 8, MoClo Format with BC fusion sites
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is the MoClo formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
Source
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD8 part off of a paper published by Mutalik, et al.