Difference between revisions of "Part:BBa K1114107"

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This part is a <a href="href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a>. From the library of BCDs, this is a MoClo version of BCD2 (BioFAB # apFAB682) and we obtained the sequence data for this part from the <a href="http://biofab.org/data">BioFAB</a>.  
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This part is a <a href="href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a>. It contains two Shine-Dalgarno sequences instead of the standard one in ribosomal binding units such as <a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. From the library of BCDs, this is a MoClo version of BCD2 (BioFAB # apFAB682) and we obtained the sequence data for this part from the <a href="http://biofab.org/data">BioFAB</a>.  
  
 
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The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
 
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
 
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<center><img src="https://static.igem.org/mediawiki/parts/1/1d/BCD_Fig1.png" width="600px"></a></center>
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This is Figure 1A from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a> showing the basic structure of their BCD design. The BCD with its two Shine-Dalgarno motifs (SD1 and SD2) is shown in gray.
  
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Revision as of 19:47, 24 September 2013

Bicistronic Design RBS 2, MoClo Format with BC fusion sites

This part is a MoClo adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from Mutalik et al., 2013. It contains two Shine-Dalgarno sequences instead of the standard one in ribosomal binding units such as BBa_B0034. From the library of BCDs, this is a MoClo version of BCD2 (BioFAB # apFAB682) and we obtained the sequence data for this part from the BioFAB.

This BCD1 is flanked by two MoClo fusion sites, B on the 5' end and C on the 3' side.

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.





This is Figure 1A from Mutalik et al., 2013 showing the basic structure of their BCD design. The BCD with its two Shine-Dalgarno motifs (SD1 and SD2) is shown in gray.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]