Difference between revisions of "Part:BBa K1114106"
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<center><img src="https://static.igem.org/mediawiki/parts/1/1d/BCD_Fig1.png" width="600px"></a></center> | <center><img src="https://static.igem.org/mediawiki/parts/1/1d/BCD_Fig1.png" width="600px"></a></center> | ||
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| − | This is Figure 1A from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a> showing the basic structure of their BCD design. The | + | This is Figure 1A from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a> showing the basic structure of their BCD design. The BCD with its two Shine-Dalgarno motifs (SD1 and SD2) is shown in gray. |
| − | its two Shine-Dalgarno motifs (SD1 and SD2)is shown in gray. | + | |
</ul></ul> | </ul></ul> | ||
Revision as of 19:46, 24 September 2013
Bicistronic Design RBS 1, MoClo Format with BC fusion sites
This part is a MoClo adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from Mutalik et al., 2013. It contains two Shine-Dalgarno sequences instead of the standard one in ribosomal binding units such as BBa_B0034. From the library of BCDs, this is a MoClo version of BCD1 (BioFAB # apFAB681) and we obtained the sequence data for this part from the BioFAB.
This BCD1 is flanked by two MoClo fusion sites, B on the 5' end and C on the 3' side.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
This is Figure 1A from Mutalik et al., 2013 showing the basic structure of their BCD design. The BCD with its two Shine-Dalgarno motifs (SD1 and SD2) is shown in gray.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]