Difference between revisions of "Part:BBa K1045016"

Revision as of 19:24, 24 September 2013

n/a	Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix

Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix

This composite part is composed of part BBa_K1045001, an inverse DarR coding sequence, fused to an inverse terminator, BBa_K1045009. DarR is a transcriptional repressor binding to the DarR operator sequence. Repression by DarR is enhanced by c-di-AMP. In E. coli, DarR seemed to act as a quite strong repressor even without c-di-AMP (Team Göttingen 2013). Part BBa_K1045016 could be cloned downstream of inverse promoter-RBS constructs (compare parts BBa_K1045010 (inverse RBS) and BBa_K1045011 (inverse promoter)) allowing it to be expressed (for example, see part BBa_K1045017).

Secondary Structure

File:Mfold-K1045016-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

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 <partinfo>BBa_K1045016 short</partinfo>
-* This composite part is composed of part BBa_K1045001, an inverse DarR coding sequence, fused to an inverse terminator, BBa_K1045009. DarR is a transcriptional repressor binding to the DarR operator sequence. Repression by DarR is enhanced by c-di-AMP. In E. coli, DarR seemed to act as a quite strong repressor even without c-di-AMP. Part BBa_K1045016 could be cloned downstream of inverse promoter-RBS constructs (compare parts BBa_K1045010 (inverse RBS) and BBa_K1045011 (inverse promoter)) allowing it to be expressed (for example, see part BBa_K1045017).
+* This composite part is composed of part BBa_K1045001, an inverse DarR coding sequence, fused to an inverse terminator, BBa_K1045009. DarR is a transcriptional repressor binding to the DarR operator sequence. Repression by DarR is enhanced by c-di-AMP. In ''E. coli'', DarR seemed to act as a quite strong repressor even without c-di-AMP (Team Göttingen 2013). Part BBa_K1045016 could be cloned downstream of inverse promoter-RBS constructs (compare parts BBa_K1045010 (inverse RBS) and BBa_K1045011 (inverse promoter)) allowing it to be expressed (for example, see part BBa_K1045017).
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