Difference between revisions of "Part:BBa K1073032"

Revision as of 18:53, 24 September 2013

Autoinducer synthase LasI + LVA with combined eforRed chromoprotein expression cassette

This part builds the basis for the construction of an expression cassette of the autoinducer synthase LasI, which synthesizes N-3-oxododecanoyl-homoserine lactone. It is combined with a expression cassette of the chromoprotein eforRed.

Usage and Biology

N-3-oxododecanoyl-HSL binds to the transcription regulator LasR. This complex then binds to specific sequences in the las promoter region activating the expression of genes located downstream (not included in this device). The constitutively expressed chromoprotein eforRed is added to the construct and can be used as a selection marker. Since this contruct does not include a promoter in front of lasI it can be combined with various regulatory regions (e.g. constitutively or regulated promoter) depending on intended use.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 838
Illegal NheI site found at 861
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 680
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 242
1000
COMPATIBLE WITH RFC[1000]

Revision as of 18:51, 24 September 2013 (view source) Laura S (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Revision as of 18:53, 24 September 2013 (view source) Laura S (Talk \| contribs) (→‎Usage and Biology) Newer edit →
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	===Usage and Biology===		===Usage and Biology===
−	N-3-oxododecanoyl-HSL binds to the transcription regulator LasR. This complex then binds to specific sequences in the las promoter region activating the expression of genes located ~~Downstream~~ (not included in this device). The constitutively expressed chromoprotein eforRed is added to the construct and can be used as a selection marker. Since this contruct does not include a promoter in front of ''lasI'' it can be combined with various regulatory regions (e.g. constitutively or regulated promoter) depending on intended use.	+	N-3-oxododecanoyl-HSL binds to the transcription regulator LasR. This complex then binds to specific sequences in the las promoter region activating the expression of genes located downstream (not included in this device). The constitutively expressed chromoprotein eforRed is added to the construct and can be used as a selection marker. Since this contruct does not include a promoter in front of ''lasI'' it can be combined with various regulatory regions (e.g. constitutively or regulated promoter) depending on intended use.