Difference between revisions of "Part:BBa K1150002"

Revision as of 17:53, 24 September 2013

KRAB

KRAB
Function	Repressor domain
Use in	Mammalian cells
RFC standard	RFC 25
Backbone	pSB1C3
Organism	Homo sapiens
Source	Konrad Müller, pKM102
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics. Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex.

Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB domains were attached to several DNA binding proteins such as tetR, TAL effectors and [http://2013.igem.org/Team:Freiburg/Project/effector#repression Cas9] - thereby efficiently silencing gene expression downstream of desired target promoters.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 244
Illegal SapI.rc site found at 208

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-Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics. Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex. <br><br> Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB domains were attached to several DNA binding proteins such as tetR, TAL effectors and <a href="http://2013.igem.org/Team:Freiburg/Project/effector#repression">Cas9</a> - thereby efficiently silencing gene expression downstream of desired target promoters.<br><br>
+Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics. Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex. <br><br> Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB domains were attached to several DNA binding proteins such as tetR, TAL effectors and [http://2013.igem.org/Team:Freiburg/Project/effector#repression Cas9] - thereby efficiently silencing gene expression downstream of desired target promoters.<br><br>
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