Difference between revisions of "Part:BBa K1150002"
Line 27: Line 27:
 
|}
 
|}
  
−
Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics. Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex. Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB domains were attached to several DNA binding proteins such as tetR, TAL effectors and Cas9 (http://2013.igem.org/Team:Freiburg/Project/effector#repression) - thereby efficiently silencing gene expression downstream of desired target promoters.
+
Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics. Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex. Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB domains were attached to several DNA binding proteins such as tetR, TAL effectors and Cas9 (http://2013.igem.org/Team:Freiburg/Project/effector#repression) - thereby efficiently silencing gene expression downstream of desired target promoters.<br><br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 33: Line 33:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'>
 +
==Sequence and Features==
 +
</span>
 
<partinfo>BBa_K1150002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1150002 SequenceAndFeatures</partinfo>
  

Revision as of 17:39, 24 September 2013

KRAB

KRAB
Function Repressor domain
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Organism Homo sapiens
Source Konrad Müller, pKM102
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics. Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex. Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB domains were attached to several DNA binding proteins such as tetR, TAL effectors and Cas9 (http://2013.igem.org/Team:Freiburg/Project/effector#repression) - thereby efficiently silencing gene expression downstream of desired target promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 244
    Illegal SapI.rc site found at 208