Difference between revisions of "Part:BBa K1073028:Experience"
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===Applications of BBa_K1073028=== | ===Applications of BBa_K1073028=== | ||
| − | The iGEM Team Braunschweig 2013 used this device for the construction of <bbpart>BBa_K1073035</bbpart>. By adding autoinducer molecules N-buturyl-HSL to the media the expression of beta lactamase can be induced. | + | The iGEM Team Braunschweig 2013 used this device in combination with <bbpart>BBa_K1073032</bbpart> for the construction of <bbpart>BBa_K1073035</bbpart>. By adding autoinducer molecules N-buturyl-HSL to the media the expression of beta lactamase can be induced. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:32, 24 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1073028
The iGEM Team Braunschweig 2013 used this device in combination with BBa_K1073032 for the construction of BBa_K1073035. By adding autoinducer molecules N-buturyl-HSL to the media the expression of beta lactamase can be induced.
User Reviews
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BBa_K1073028 iGEM Team Braunschweig 2013 |
This device was used for the construction of BBa_K1073035. With this construct in a high copy plasmid (pSB1C3) we were able to induce expression of beta lactamase in E. coli Top10 when N-buturyl-HSL was added to the media and thus maintain cells growth in complex medium containing ampicillin. For detailed experience see BBa_K1073035 |
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