Difference between revisions of "Part:BBa K1031744"

 
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114-PaaX-TT
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== '''Construction''' ==
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In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031744 is composed of three elements, the constitutive ''Pc'' promoter J23114[https://parts.igem.org/Part:BBa_J23114], coding sequence of PaaX and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/e/e0/Peking2013_part_114-PaaX-TT.png/800px-Peking2013_part_114-PaaX-TT.png", width=600px; />
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'''Fig 1''' Construction of biosensor circuit ''Pa''/J23114-PaaX
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== '''Sequence and Features''' ==
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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Revision as of 17:06, 24 September 2013

J23114-PaaX


Construction

In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031744 is composed of three elements, the constitutive Pc promoter J23114[1], coding sequence of PaaX and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit Pa/J23114-PaaX


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]