Difference between revisions of "Part:BBa K1031112"

Revision as of 15:11, 24 September 2013

J23113-CapR-Terminator

Characterization

In order to fine-tune the performance of CapR biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive Pc promoter J23113[1], coding sequence of CapR and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit Po/J23113-CapR

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 147
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 344
Illegal NgoMIV site found at 625
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 851
Illegal SapI.rc site found at 1508

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1031112 short</partinfo>
--CapR-TT
+<!-- -->
+== '''Characterization''' ==
+In order to fine-tune the performance of CapR biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive ''Pc'' promoter J23113[https://parts.igem.org/Part:BBa_J23113], coding sequence of CapR and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
+<html>
+<img src="https://static.igem.org/mediawiki/igem.org/thumb/a/af/113-CapR-TT.png/800px-113-CapR-TT.png", width=600px; />
+</html>
+'''Fig 1''' Construction of biosensor circuit ''Po''/J23113-CapR
+== '''Sequence and Features''' ==
-<!-- Add more about the biology of this part here
-===Usage and Biology===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1031112 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K1031112 parameters</partinfo>
+<partinfo>BBa_K1031912 parameters</partinfo>
 <!-- -->