Difference between revisions of "Part:BBa K1031112"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1031112 short</partinfo>
 
<partinfo>BBa_K1031112 short</partinfo>
  
113-CapR-TT
+
<!-- -->
 +
 
 +
 
 +
== '''Characterization''' ==
 +
 
 +
 
 +
In order to fine-tune the performance of CapR biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive ''Pc'' promoter J23113[https://parts.igem.org/Part:BBa_J23113], coding sequence of CapR and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
 +
 
 +
<html>
 +
<img src="https://static.igem.org/mediawiki/igem.org/thumb/a/af/113-CapR-TT.png/800px-113-CapR-TT.png", width=600px; />
 +
</html>
 +
 
 +
'''Fig 1''' Construction of biosensor circuit ''Po''/J23113-CapR
 +
 
 +
 
 +
== '''Sequence and Features''' ==
  
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1031112 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031112 SequenceAndFeatures</partinfo>
 +
 +
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
<partinfo>BBa_K1031112 parameters</partinfo>
+
<partinfo>BBa_K1031912 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 15:11, 24 September 2013

J23113-CapR-Terminator


Characterization

In order to fine-tune the performance of CapR biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031111 is composed of three elements, the constitutive Pc promoter J23113[1], coding sequence of CapR and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit Po/J23113-CapR


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 344
    Illegal NgoMIV site found at 625
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 851
    Illegal SapI.rc site found at 1508