Difference between revisions of "Part:BBa C0051:Design"
(→‎Design Notes)
Line 19: Line 19:
 
<a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization      of CI repressor is needed for effective repression of PRM and efficient switching      from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and      J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001   
 
<a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization      of CI repressor is needed for effective repression of PRM and efficient switching      from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and      J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001   
  
−
BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator.      It has been modified to include a rapid degradation LAA tail, and includes      the BioBrick standard assembly head and tail restriction sites. The RBS has      been removed. The stop codon has been changed from TAA to a double stop codon      TAATAA.
+
BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator.      It has been modified to include a rapid degradation LVA tail, and includes      the BioBrick standard assembly head and tail restriction sites. The RBS has      been removed. The stop codon has been changed from TAA to a double stop codon      TAATAA.
−
 
+
−
 
+
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 05:01, 15 July 2006


cI repressor from E. coli phage lambda (+LVA)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

References (unparsed) here:

<a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000

<a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001

References (unparsed) here:

<a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000

<a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001

BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.

Source

Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).

References