Difference between revisions of "Part:BBa K1031916"
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In order to fine-tune the performance of XylS biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031912 is composed of three elements, the constitutive ''Pc'' promoter J23105[https://parts.igem.org/Part:BBa_J23105], coding sequence of XylS and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
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In order to fine-tune the performance of XylS biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031916 is composed of three elements, the constitutive ''Pc'' promoter J23114[https://parts.igem.org/Part:BBa_J23114], coding sequence of XylS and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
  
 
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'''Fig 1''' Construction of biosensor circuit ''Pm''/J23105-XylS
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'''Fig 1''' Construction of biosensor circuit ''Pm''/J23114-XylS
  
  

Revision as of 12:04, 24 September 2013

J23114-XylS-Terminator


Characterization

In order to fine-tune the performance of XylS biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031916 is composed of three elements, the constitutive Pc promoter J23114[1], coding sequence of XylS and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit Pm/J23114-XylS


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 93
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 278


Data shown

Induction ratio of XylS biosensor library from ON/OFF test that utilizes different Pc promoters to control the expression of XylS. The dashed box refers to data for Pm/J23114-XylS biosensor circuit (Fig 2) This figure shows the biosensor circuit adopting J23114 obtains optimal performance.

Fig 2 Horizontal axis stands for different XylS biosensor with Pc promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Partsregistry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following Test Protocol 1 [1]. Vertical axis represents the ON-OFF induction ratio. The dashed box refers to performance for Pm/J23114-XylS biosensor circuit.