Difference between revisions of "Part:BBa K1150033:Experience"
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===Applications of BBa_K1150033=== | ===Applications of BBa_K1150033=== | ||
| − | + | GFP expression | |
===User Reviews=== | ===User Reviews=== | ||
| + | Team Heidelberg received this part from Team Freiburg to establish Gibson-assembly cloning in the Heidelberg lab. After a successful four-fragment Gibson (backbone: pSB1C3, CMV promoter, acGFP and BGH terminator) chemical and electrical E.Coli were transformed and analyzed two days later by fluorescence microscopy. A high fluorescence intensity could be detected. | ||
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Latest revision as of 09:42, 24 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1150033
GFP expression
User Reviews
Team Heidelberg received this part from Team Freiburg to establish Gibson-assembly cloning in the Heidelberg lab. After a successful four-fragment Gibson (backbone: pSB1C3, CMV promoter, acGFP and BGH terminator) chemical and electrical E.Coli were transformed and analyzed two days later by fluorescence microscopy. A high fluorescence intensity could be detected. UNIQ1d5f21a1178f5ff0-partinfo-00000000-QINU UNIQ1d5f21a1178f5ff0-partinfo-00000001-QINU