Difference between revisions of "Part:BBa K1114705"

 
 
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This is a BioBrick adaptation of a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="https://parts.igem.org/Part:BBa_K1114111:Design">BCD16_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="https://parts.igem.org/Part:BBa_K1114300">B0015_DE</a> within the <a href="https://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
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This is a BioBrick adaptation of a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="https://parts.igem.org/Part:BBa_K1114111:Design">BCD16_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="https://parts.igem.org/Part:BBa_K1114300">B0015_DE</a> within the <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
 
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
 
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
  

Latest revision as of 04:53, 24 September 2013

Terminator.png

BioBrick adaptation of MoClo Level 1 RFP Reporter

  • This is a BioBrick adaptation of a Level 1 MoClo device that constitutively expresses RFP. It contains the Level 0 parts J23104_AB, BCD16_BC, E1010m_CD, and B0015_DE within the pSB1C3 backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.


K1114005

K1114111

K1114211

K1114300

Secondary Structure

File:Mfold-K1114705-1.png


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]