Difference between revisions of "Part:BBa K1140005:Design"

(→‎Design Notes)
(→‎Source)
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Obtained synthetically.
 
Obtained synthetically.
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The RBS sequence is derived from part BBa_B0034, which features in the 2010 study by Elowtiz and Leibler. This RBS is defined as the standard for RBS activity and is assigned an efficiency of 1.0.
+
The RBS sequence is derived from part BBa_B0034, which features in the 2000 study by Elowtiz and Leibler. This RBS is defined as the standard for RBS activity and is assigned an efficiency of 1.0.
−
The coding sequence of TetR was derived from part BBa_C0040.  
+
The coding sequence of TetR was derived from part BBa_C0040.
  
 
===References===
 
===References===
 
Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, ''Nature'', 20;403(6767):335-8.
 
Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, ''Nature'', 20;403(6767):335-8.

Revision as of 04:53, 24 September 2013

RBS + TetR (LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.

Source

Obtained synthetically. The RBS sequence is derived from part BBa_B0034, which features in the 2000 study by Elowtiz and Leibler. This RBS is defined as the standard for RBS activity and is assigned an efficiency of 1.0. The coding sequence of TetR was derived from part BBa_C0040.

References

Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, Nature, 20;403(6767):335-8.