Difference between revisions of "Part:BBa K1140005:Design"
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===Design Notes=== | ===Design Notes=== | ||
The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI. | The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI. | ||
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===Source=== | ===Source=== | ||
Revision as of 04:52, 24 September 2013
RBS + TetR (LVA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.
Source
Obtained synthetically. The RBS sequence is derived from part BBa_B0034, which features in the 2010 study by Elowtiz and Leibler. This RBS is defined as the standard for RBS activity and is assigned an efficiency of 1.0. The coding sequence of TetR was derived from part BBa_C0040.
References
Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, Nature, 20;403(6767):335-8.