Difference between revisions of "Part:BBa K1114700"

Revision as of 04:50, 24 September 2013

Level 1 MoClo device that constitutively expresses mCitrine

This is a BioBrick adaptation of a Level 1 MoClo device that constitutively expresses the fluorescent protein mCitrine. It contains the Level 0 parts J23104_EB, BCD2_BC, mCitrine_CD, and B0015_DE within the pSB1C3 backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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−	This is a BioBrick adaptation of a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses the fluorescent protein mCitrine. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="https://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114214">mCitrine_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783020">B0015_DE</a> within the <a href="https://parts.igem.org/Part:~~BBa_K1114419~~">~~DVL1_EF~~</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.	+	This is a BioBrick adaptation of a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses the fluorescent protein mCitrine. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="https://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114214">mCitrine_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783020">B0015_DE</a> within the <a href="https://parts.igem.org/Part:BBa_pSB1C3">pSB1C3</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
	The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.		The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.