Difference between revisions of "Part:BBa I15010:Design"

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===References===
 
===References===
  
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* [Gambetta and Lagarias. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10566-71 www.pnas.org/cgi/doi/10.1073/pnas.191375198].
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* [http://www.pnas.org/cgi/doi/10.1073/pnas.191375198 Gambetta and Lagarias. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10566-71].

Latest revision as of 16:41, 14 July 2006


cph8 (Cph1/EnvZ fusion)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2257
    Illegal XhoI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutation at base 108 (G-A) to remove PstI site


Source

Voigt Lab via Anselm Levskaya

References

  • [http://www.pnas.org/cgi/doi/10.1073/pnas.191375198 Gambetta and Lagarias. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10566-71].