Difference between revisions of "Part:BBa I15010:Design"
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− | * Gambetta and Lagarias. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10566-71. | + | * [Gambetta and Lagarias. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10566-71 www.pnas.org/cgi/doi/10.1073/pnas.191375198]. |
Revision as of 16:38, 14 July 2006
cph8 (Cph1/EnvZ fusion)
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2257
Illegal XhoI site found at 364 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Silent mutation at base 108 (G-A) to remove PstI site
Source
Voigt Lab via Anselm Levskaya
References
- [Gambetta and Lagarias. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10566-71 www.pnas.org/cgi/doi/10.1073/pnas.191375198].