Difference between revisions of "Part:BBa K1073013"

Revision as of 18:52, 23 September 2013

Inducible ampicillin resistance cassette (regulated by LasR + N-3-oxododecanoyl-HSL)

This device is a combination of BBa_K1073003 and BB_K1073000 and mediates a inducible ampicillin resistance. It includes the inducible promoter of the las quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.

Usage and Biology

The promoter is activated in the presence of LasR in combination with the autoinducer molecule N-3-oxododecanoyl-homoserine lactone produced by LasI. The expression of the beta-lactamase can therefore be induced and tuned by supplying LasR and N-3-oxododecanoyl-HSL.

Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073034.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-The promoter for this beta-lactonase is induced by N-(3-oxododecanoyl)-homoserine lactone. Therefore the expression of the ampicillin beta-lactonase is inducable by Las inducer N-(3-oxododecanoyl)-homoserine lactone.
 The promoter is activated in the presence of LasR in combination with the autoinducer molecule N-3-oxododecanoyl-homoserine lactone produced by LasI. The expression of the beta-lactamase can therefore be induced and tuned by supplying LasR and N-3-oxododecanoyl-HSL.