Difference between revisions of "Part:BBa K1073009"

Revision as of 17:51, 23 September 2013

LasI synthetase + LVA with subsequent double terminator

This construct is derived from BBa_C0078 and B0015. It combines the autoinducer synthetase LasI (producing N-oxo-dodecanoyl-HSL)including the LVA degradation tag with a double terminator to prevent further transciption.

Usage and Biology

The synthetase LasI produces the autoinducer molecule N-oxo-dodecanoyl-HSL of the Las Quorum sensing system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 661
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 223
1000
COMPATIBLE WITH RFC[1000]

Revision as of 17:50, 23 September 2013 (view source) Kerstin K (Talk \| contribs) ← Older edit		Revision as of 17:51, 23 September 2013 (view source) Kerstin K (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K1073009 short</partinfo>		<partinfo>BBa_K1073009 short</partinfo>

−	This construct is derived from BBa_C0078 and B0015. It combines the autoinducer synthetase LasI (producing N-oxo-dodecanoyl-HSL) with a double terminator to prevent further transciption.	+	This construct is derived from BBa_C0078 and B0015. It combines the autoinducer synthetase LasI (producing N-oxo-dodecanoyl-HSL)including the LVA degradation tag with a double terminator to prevent further transciption.