Difference between revisions of "Part:BBa K1065310:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1065310 short</partinfo>
 
<partinfo>BBa_K1065310 short</partinfo>
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===Design Notes===
 
===Design Notes===
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We created this part by putting a costitutive promoter (J23100) in front of a blue light receptor protein. Then we put this construct in the K592020 part.
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we designed this part by inserting an Anderson promoter(J23100) upstream Bba_K592016 (YF1 and FixJ) creating a new part: Bba_K1065306. then we combined this part with BBa_K592020 (that consists of the rest of the circuit, the inverter cassette and the reporter amilCP.<BR>
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Note that a terminator is missing after PfixK2. We suggest to instert at least one Terminater after the inducible promoter in order to have a better defined switch from the ON state to the OFF state.
  
  
 
===Source===
 
===Source===
  
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This part was obtained by combining many parts together.
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This part was obteined using three parts:<BR>
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Bba_J23100<BR>
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===References===
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Bba_K592016<BR>
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BBa_K592020<BR>

Revision as of 13:58, 23 September 2013

Blue light circuit with inverter for the production of amilCP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


Design Notes

we designed this part by inserting an Anderson promoter(J23100) upstream Bba_K592016 (YF1 and FixJ) creating a new part: Bba_K1065306. then we combined this part with BBa_K592020 (that consists of the rest of the circuit, the inverter cassette and the reporter amilCP.
Note that a terminator is missing after PfixK2. We suggest to instert at least one Terminater after the inducible promoter in order to have a better defined switch from the ON state to the OFF state.


Source

This part was obteined using three parts:
Bba_J23100
Bba_K592016
BBa_K592020